Bacterial surface display systems have been developed based on non-pathogenic Staphylococcus species (1,2,3). The report from 1992 (1) was the first report on surface display on a Gram-positive bacterium. The devised host vector systems for efficient surface display on food grade staphylococci, e.g. S. carnosus, have been used successfully for subunit vaccine delivery (8,12,16), or as whole cell tools for diagnostic (5,13) or environmental applications (15,18). The present focus is to evaluate staphylococci for protein library applications using fluorescence-activated cell sorting (FACS) for library screening. Two recent scientific achievements have been reached: (i) the vector system have been optimized to yield genetically stable vectors to allow library applications (19), and (ii) in a simulated library selection experiment, a more than 25,000-fold enrichment of staphylococcal target cells have been demonstrated (22). The obtained results indicate that staphylococcal surface display of protein libraries combined with FACS sorting might indeed constitute an attractive alternative to existing technology platforms for high-throughput affinity based selections.