| Year |
Research
field |
Description |
Key reference |
| 2005 |
Human
Protein Atlas |
A publicly available database
with 400,000 images (www.proteinatlas.org) |
Uhlén et al (2005) Mol
Cell Proteomics in press (e publ) |
| 2004 |
Whole
genome sequencing |
The complete genome of Mycoplasma
mycoides was determined and annotated |
Westberg et al (2004) Genome
Research 14(2), 221-227 |
| 2003 |
Antibody
Proteomics |
The first description of high-throughput
antibody-based tissue profiling |
Agaton et al (2003) Mol. Cell.
Prot. 2: p. 405-414 |
| 2001 |
Tree
genome discovery |
A transcriptional roadmap to
wood formation by microarray analysis (collaboration with
UmU/SLU) |
Hertzberg et al. (2001) Proc
Natl Acad Sci U S A 98, 14732-37 |
| 2000 |
Stabilization
of proteins |
Recombinant proteins can be
engineered to tolerate pH above 13 |
Gulich et al (2000) J Biotech
80: 169-178 |
| 1998 |
Pyrosequencing |
A new DNA sequencing method
(collaboration with P. Nyren) |
Ronaghi et al (1998) Science
281: 363-365 |
| 1997 |
Analysis
of single cells |
First report on DNA sequence
analysis of micro dissected single cancer cells |
Ponten et al (1997) Mutat Res
1: 45-55 |
| 1997 |
Affibodies |
Robust affinity reagents selected
from combinatorial libraries |
Nord et al (1997) Nature Biotechno-logy
15: 772-777 |
| 1995 |
Biosensor
analysis of DNA manipulations |
The first report on real-time
biosensor analysis of DNA manipulations |
Nilsson et al (1995) Anal Biochem
224: 400-408 |
| 1992 |
Bioautomation
of magnetic separations |
Robotics for sample preparation
based on magnetic handling |
Uhlen et al (1992) Trends in
Biotechnol. 10: 52-55 |
| 1992 |
Gram+
bacterial surface display |
The first report on the use
of Gram positive bacteria for surface expression of recombinat
proteins |
Hansson et al (1992) J. Bacteriol.
174: 4239-4245 |
| 1991 |
Affinity-based
folding and purification |
First report on the use of affinity
principles to facilitate in vivo folding of recombinant
proteins |
Samuelsson et al (1991) Nature
Biotechn 9: 363-366 |
| 1991 |
In
vivo stabilization of recombinant proteins |
The half-life of recombinant
proteins in circulation can be increased by fusion to an
albumin-binding domain |
Nygren et al (1991) Vaccines
91: 363-368 |
| 1988 |
Solid
phase sequencing and DNA diagnostics |
The first publication on solid
phase sequencing and the use of streptavidin coated magnetic
beads |
Stahl et al (1988) Nucl Acids
Res 16: 3025-3038 |
| 1986 |
Recombinant
protein G |
Protein G is used for purification
of antibodies, antibody fragments and human serum albumin |
Guss et al. (1986) EMBO J 5:
1567-1575 |
| 1984 |
Recombinant
protein A |
Protein A is used extensively
as affinity ligand for purification of antibodies |
Uhlen et al. (1984) J Biol Chem
259: 1695-1702 |
| 1983 |
Gene
fusions for affinity purification of proteins |
The protein A gene fusion system
was the first published report for the use of affinity tags
for purification |
Uhlen et al (1983) Gene 23:
369-378 |
