School of Biotechnology


Mathias Uhlen


KTH Biotechnology
AlbaNova University Center
Royal Institute of Technology (KTH)
106 91 Stockholm, Sweden

Direct phone: +46 (0) 8 5537 8325
Secr: +46 (0) 8 5537 8403
Fax: +46 8 5537 8482


10 selected innovations

Short Biography

Mathias Uhlen is Professor of Microbiology at the Royal Institute of Technology (KTH), Stockholm, Sweden. Dr Uhlen is member of the Royal Swedish Academy of Engineering Science (IVA), the Royal Swedish Academy of Science (KVA), the European Molecular Biology Organization (EMBO) and member of the Human Proteome Organization (HUPO) council. He was Vice-President of the Royal Institute of Technology (KTH), responsible for external relations, from 1999 to 2001. He was the chairman of the Swedish Biochemical and Molecular Biology Society (SFMB) from 1994 to 1999. Dr Uhlen has more than 300 publications in bioscience with the focus on the development and use of affinity reagents in biotechnology and biomedicine.

In the early eighties, Dr Uhlen cloned and characterized staphylococcal protein A, which is now used extensively for purification of antibodies both in diagnostics and therapy. He also showed in 1983 that protein A could be used as an affinity tag for purification of other proteins. The use of affinity tags for purification of recombinant proteins are now widely used in bioscience. In the late eighties, Uhlen published the use of magnetic micro spheres with streptavidin for automated solid phase applications. Such laboratory systems based on streptavidin beads are at present frequently used both in research and diagnostics. In the 90:ies, his group described a new principle for affinity reagents, called Affibodies, and showed their use as research tool and recently as potential cancer therapeutics. Uhlen and colleagues also developed a new strategy for DNA analysis called Pyrosequencing, a method that has recently been further developed by a US company into a highly parallelized sequencing instrument.
Dr Uhlen is currently working on the Human Protein Resource Project (HPR), with the aim to systematically map the human proteome. At present, the Human Protein Atlas portal ( contains more than 7 million high-resolution images representing 6,800 human proteins.
He has founded several companies, including Pyrosequencing AB (now Biotage AB), Affibody AB, SweTree Technologies AB, Magnetic Biosolutions AB (now Nordiag AS), Atlas Antibodies AB and Creative Peptides AB. He has received numerous awards, including The Svedberg prize in 1992, the Göran Gustavsson prize in 1993, the gold medal of the Royal Swedish Academy of Engineering Sciences in 2004, the Most Noble Order of the Seraphim - the Order of His Majesty the King in 2004, the Jerker Porath award in 2005, the Akzo Noble Award in 2005, the HUPO Distinguished Award and KTH Great Prize both in 2006 and the Scheele prize in 2007.
Last updated: 2013-03-06