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Principal Investigator (PI): Prof Mathias Uhlen
Personnel: Inger Åhlén (secretary),
Lisa Berglund (PhD-student) , Anders Holmberg (PhD-student),
Mats Lindskog (PhD-student) and Johan Rockberg (PhD-student)
Description: The major focus of this group
is in the field of Antibody Proteomics as part of the Swedish
Human Proteomics Resource (HPR). A systematic approach for
production of recombinant Protein Epitope Signature Tags (PrESTs)
for the generation of antibodies has been developed. This
approach can be used to generate and enrich mono-specific
antibodies for systematic analysis of protein profiles in
different human organs using tissue arrays. This strategy
is used to produce a proteome atlas, describing distribution
and expression of human proteins in normal tissues as well
as in common cancers and other forms of diseased tissues.
In addition, a high-throughput system for analysis of single
nucleotide polymorphisms (SNP) has been developed based on
pyrosequencing. The sample preparation is based on “in-house”
designed robotic workstations that have been developed to
allow automated sample handling in the fields of genomics,
transcript profiling and proteomics. The automated sequencing
procedure has been used to determine the complete genome sequence
of Mycoplasma mycoides, a veterinary pathogen.
| Recent publications: |
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Nilsson P, Paavilainen L, Larsson
K, Odling J, Sundberg M, Andersson AC,
Kampf C, Persson A, Szigyarto CA, Ottosson J, Bjorling E,
Hober S, Wernerus H,
Wester K, Ponten F, Uhlen M. (2005) “Towards a human
proteome atlas: High-throughput generation of mono-specific
antibodies for tissue profiling” Proteomics. 5(17):4327-37 |
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Janzi M, Odling J, Pan-Hammarstrom Q, Sundberg
M, Lundeberg J, Uhlen M,
Hammarstrom L, Nilsson P. (2005) “Serum Microarrays
for Large Scale Screening of Protein Levels “ Mol
Cell Proteomics. 4(12):1942-1947. Epub 2005 Aug 29 |
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Uhlen et al (2005) “A Human Protein
Atlas for Normal and Cancer Tissues Based on Antibody Proteomics”
Mol Cell Proteomics. 4(12):1920-1932. Epub 2005 Aug 27. |
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Stenvall M, Steen J, Uhlen M, Hober S, Ottosson
J. (2005) “High-throughput solubility assay for purified
recombinant protein immunogens” Biochim Biophys Acta.
31;1752(1):6-10 |
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Hedhammar M, Stenvall M, Lonneborg R, Nord
O, Sjolin O, Brismar H, Uhlen M,
Ottosson J, Hober S. (2005) “A novel flow cytometry-based
method for analysis of expression levels in Escherichia
coli, giving information about precipitated and soluble
protein” J Biotechnol. 23;119(2):133-46 |
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Lindskog M, Rockberg J, Uhlen M, Sterky F.
(2005) “Selection of protein epitopes for antibody
production” Biotechniques. 2005 38(5):723-7 |
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Uhlen M, Ponten F. (2005) “Antibody-based
proteomics for human tissue profiling” Mol Cell Proteomics.
4(4):384-93. Epub 2005 Feb 5 |
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Holmberg A, Blomstergren A, Nord O, Lukacs
M, Lundeberg J, Uhlen M. (2005) “The biotin-streptavidin
interaction can be reversibly broken using water at
elevated temperatures” Electrophoresis. 26(3):501-10 |
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Agaton C, Falk R, Hoiden Guthenberg I, Gostring
L, Uhlen M, Hober S. (2005) “Selective enrichment
of monospecific polyclonal antibodies for antibody-based
proteomics efforts” J Chromatogr A. 16;1043(1):33-40 |
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