School of Biotechnology

  High-throughput Proteomics and Functional Genomics
Al-Khalili Szigyarto

Principal Investigator (PI): Prof Mathias Uhlen

Personnel: Inger Åhlén (secretary), Lisa Berglund (PhD-student) , Anders Holmberg (PhD-student), Mats Lindskog (PhD-student) and Johan Rockberg (PhD-student)

Description: The major focus of this group is in the field of Antibody Proteomics as part of the Swedish Human Proteomics Resource (HPR). A systematic approach for production of recombinant Protein Epitope Signature Tags (PrESTs) for the generation of antibodies has been developed. This approach can be used to generate and enrich mono-specific antibodies for systematic analysis of protein profiles in different human organs using tissue arrays. This strategy is used to produce a proteome atlas, describing distribution and expression of human proteins in normal tissues as well as in common cancers and other forms of diseased tissues. In addition, a high-throughput system for analysis of single nucleotide polymorphisms (SNP) has been developed based on pyrosequencing. The sample preparation is based on “in-house” designed robotic workstations that have been developed to allow automated sample handling in the fields of genomics, transcript profiling and proteomics. The automated sequencing procedure has been used to determine the complete genome sequence of Mycoplasma mycoides, a veterinary pathogen.

Recent publications:
Nilsson P, Paavilainen L, Larsson K, Odling J, Sundberg M, Andersson AC,
Kampf C, Persson A, Szigyarto CA, Ottosson J, Bjorling E, Hober S, Wernerus H,
Wester K, Ponten F, Uhlen M. (2005) “Towards a human proteome atlas: High-throughput generation of mono-specific antibodies for tissue profiling” Proteomics. 5(17):4327-37
Janzi M, Odling J, Pan-Hammarstrom Q, Sundberg M, Lundeberg J, Uhlen M,
Hammarstrom L, Nilsson P. (2005) “Serum Microarrays for Large Scale Screening of Protein Levels “ Mol Cell Proteomics. 4(12):1942-1947. Epub 2005 Aug 29
Uhlen et al (2005) “A Human Protein Atlas for Normal and Cancer Tissues Based on Antibody Proteomics” Mol Cell Proteomics. 4(12):1920-1932. Epub 2005 Aug 27.
Stenvall M, Steen J, Uhlen M, Hober S, Ottosson J. (2005) “High-throughput solubility assay for purified recombinant protein immunogens” Biochim Biophys Acta. 31;1752(1):6-10
Hedhammar M, Stenvall M, Lonneborg R, Nord O, Sjolin O, Brismar H, Uhlen M,
Ottosson J, Hober S. (2005) “A novel flow cytometry-based method for analysis of expression levels in Escherichia coli, giving information about precipitated and soluble protein” J Biotechnol. 23;119(2):133-46
Lindskog M, Rockberg J, Uhlen M, Sterky F. (2005) “Selection of protein epitopes for antibody production” Biotechniques. 2005 38(5):723-7
Uhlen M, Ponten F. (2005) “Antibody-based proteomics for human tissue profiling” Mol Cell Proteomics. 4(4):384-93. Epub 2005 Feb 5
Holmberg A, Blomstergren A, Nord O, Lukacs M, Lundeberg J, Uhlen M. (2005) “The biotin-streptavidin interaction can be reversibly broken using water at
elevated temperatures” Electrophoresis. 26(3):501-10
Agaton C, Falk R, Hoiden Guthenberg I, Gostring L, Uhlen M, Hober S. (2005) “Selective enrichment of monospecific polyclonal antibodies for antibody-based
proteomics efforts” J Chromatogr A. 16;1043(1):33-40
Last updated: 2013-03-06