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We have described (1,2,3 and 4) a strategy for high-throughput
generation of specific antibodies towards recombinant proteins.
Recently, this approach has been further developed with regard
to (i) bioinformatics algorithms that have allowed the identification
of coding sequences that are amenable both to recombinant
protein expression and the generation of specific antibody
reagents, (ii) a robust E. coli recombinant protein expression
system, (iii) systematic antibody production associated with
antigen purification of polyclonal sera (5) and, (iv) a systematic
approaches to utilizing these reagents, in particular using
tissue arrays (unpublished). The project has led to the initiation
of a Human Proteome Resource
program funded by Knut and Alice Wallenberg Foundation.
| Key (own)
publications: |
| 1. |
Larsson, M., E. Brundell, L.
Nordfors, C. Hoog, M. Uhlen, and S. Stahl (1996) A general
bacterial expression system for functional analysis of cDNA-encoded
proteins. Protein Expr Purif 7, 447-457. |
| 2. |
Larsson, M., S. Graslund, L. Yuan, E. Brundell,
M. Uhlen, C. Hoog, and S. Stahl (2000) High-throughput protein
expression of cDNA products as a tool in functional genomics.
J Biotechnol 80, 143-157. |
| 3. |
Gräslund et al A high-stringency proteomics
concept aimed for generation of antibodies specific for
cDNA-encoded proteins. Biotechnol. Applied Biochem. 35:
75-82 (2002). |
| 4. |
Agaton, C., J. Galli, I. Hoiden Guthenberg,
L. Janzon, M. Hansson, A. Asplund, E. Brundell, S. Lindberg,
I. Ruthberg, K. Wester, D. Wurtz, C. Hoog, J. Lundeberg,
S. Stahl, F. Ponten, and M. Uhlen (2003) Affinity proteomics
for systematic protein profiling of chromosome 21 gene products
in human tissues. Mol Cell Proteomics, 2(6): p. 405-14. |
5. |
Agaton, C., R. Falk, I. Höjden-Guthenberg,
L. Göstring, M Uhlen and S. Hober, Selective enrichement
of monospecific antibodies for antibody-based proteomics,
J. Chromatogr. 2004, 1043, 33-40. |
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