Principal Investigator (PI): Dr
Fredrik Sterky
Personnel: Per Unneberg, Michael Strömberg
, Mats Lindskog (with Mathias Uhlen)
Description: This group focus on high-throughput
DNA sequencing and genotyping as well as the development of
software tools for data mining, biodiversity studies and proteomics.
To date, 100 000 gene fragments have been produced from 19
tissues of poplar trees. This extensive data set is the basis
for further analysis using microarray technology and the clone
bank is an important resource for detailed analysis of the
most interesting genes. Data mining efforts and database development
enables collaborating research groups to access the information
for further studies focused on the biology and genetics behind
wood development and other basic mechanisms of the tree. At
present, the work at KTH are focussed on three main projects:
1) Development of advanced algorithms and software for SNP
discovery, 2) SNP analysis in aspen (Populus tremula) and
3) the production of antibodies against selected genes related
to wood formation.
| Selected
publications: |
| • |
Sterky et al. (2004) “A
Populus EST resource for plant functional genomics”
PNAS (in press) |
| • |
Andersson et al. (2004) “A transcriptional
timetable of autumn senescence” Genome Biology 5(4):R24 |
| • |
Finkelstein et al. (2002) “Microarray
data quality analysis: lessons from the AFGC project. Arabidopsis
Functional Genomics Consortium” Plant Mol Biol 48:119-131. |
| • |
Sterky et al. (2001) “Tree genomics
in Sweden: a national network of biological and technological
expertise” AgBiotechNet, 3:1-5. |
• |
Sterky, F. and Lundeberg, J. (2000) “Sequence
analysis of genes and genomes” Journal of Biotechnology,
76:1-31. |
| • |
Sterky et al. (1998) ”Gene discovery
in the wood-forming tissues of poplar: analysis of 5,692
expressed sequence tags” Proc Natl Acad Sci U S A
95:13330-13335. |
