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Principal Investigator (PI):
Dr Peter Nilsson
Personnel: Annelie Waldén, (research
engineer), Jenny Ödling (research engineer), Rebecca
Rimini (researcher), Angela Fredin (technician), Cecilia Laurell
(PhD student, with Joakim Lundeberg), Esther Edlundh-Rose
(PhD student, with Joakim Lundeberg), Johan Lindberg (PhD
student, with Joakim Lundeberg), Marcus Gry Björklund
(PhD student, with Joakim Lundeberg), Hanna Gräns (PhD
student, with Birgitta Evengård, KI)
Description: The reasarch is based on the
activities at the KTH microarray
resource center, with focus on methodology and technology
development of all aspects of DNA and protein microarray related
activities, such as large-scale array production, high-throughput
target and probe preparation, automation, amplification protocols,
as well as several of the bioinformatic tools needed for a
microarray analysis. The method and technology driven research
is generally applied to a wide variaty of biological research
projects together with collaborators, such as transcript profiling
in cancer related projects, Chronic Fatigue Syndrome, Archaea
and Poplar. Recently has also projects with different formats
of protein microarrays been initiated, applied to analysis
of antibody specificity and screening of clinical samples.
| Selected
recent publications: |
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Gräns, H, Nilsson, P, and Evengård,
B. Gene expression profiling of Swedish patients with Chronic
Fatigue Syndrome. J Internal Medicin, 2005 in press. |
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Janzi, M, Ödling, J, Pan-Hammarström,
Q, Sundberg, M, Lundeberg, J, Uhlen, M, Hammarström,
L, and Nilsson, P. Serum microarrays for large scale screening
of protein levels. Mol Cell Proteomics, 2005 in press. |
| • |
Nilsson, P, Paavilainen, L, Larsson, K, Sundberg,
M, Ödling, J, Andersson, A-C, Kampf, C, Persson, A,
Szigyarto, CA-K, Ottosson, J, Björling, E, Hober, S,
Wernérus, H, Wester, K, Pontén, F, and Uhlen,
M. Towards a human proteome atlas: high-throughput generation
of mono-specific antibodies for tissue profiling. Proteomics,
2005 in press. |
| • |
Nordström, H, Falk, K, Lindegren, G,
Mouzavi-Jazi, M, Johansson, A, Elgh, F, Nilsson, P, and
Lundkvist, Å. Microarray based detection and identification
of seven pathogenic flaviviruses. J Med Virol, 2005 in press. |
• |
Velazquez-Fernandez, D, Laurell, C, Geli,
J, Höög, A, Odeberg, J, Kjellman, M, Lundeberg,
J, Hamberger, B, Nilsson, P, and Bäckdahl, M. Expression
profiling of adrenocortical tumors suggests a molecular
signature of malignancy. Surgery, 2005 in press. |
| • |
Andersson, A, Bernander, R, and Nilsson,
P. Dual-genome primer design for construction of DNA microarrays.
Bioinformatics, 2005. 21(3): 325-332. |
• |
Edlundh-Rose, E, Kupershmidt, I, Gustafsson,
A, Parasassi, T, Bracci-Laudiero, L, Greco, G, Krasnowska,
E, Romano, M, Lundeberg, T, Nilsson, P, and Lundeberg, J.
Gene expression analysis of human epidermal keratinocytes
after N-acetyl L-cysteine treatment demonstrates cell cycle
arrest and increased differentiation. Pathobiology, 2005.
72(4): 203-212. |
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Lönnstedt, IM, Rimini, R, and Nilsson,
P. Bayesian microarray one-way anova and grouping cell lines
by equal expression levels. Statistical Applications in
Genetics and Molecular Biology, 2005. 4(1): Article 7. |
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Andersson, A, Keskitalo, J, Sjodin, A, Bhalerao,
R, Sterky, F, Wissel, K, Tandre, K, Aspeborg, H, Moyle,
R, Ohmiya, Y, Brunner, A, Gustafsson, P, Karlsson, J, Lundeberg,
J, Nilsson, O, Sandberg, G, Strauss, S, Sundberg, B, Uhlen,
M, Jansson, S, and Nilsson, P. A transcriptional timetable
of autumn senescence. Genome Biol, 2004. 5(4): R24. |
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Lundgren, M, Andersson, A, Chen, L, Nilsson,
P, and Bernander, R. Three replication origins in Sulfolobus
species: synchronous initiation of chromosome replication
and asynchronous termination. Proc Natl Acad Sci U S A,
2004. 101(18): 7046-51. |
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Nordström, H, Johansson, P, Li, QG,
Lundkvist, A, Nilsson, P, and Elgh, F. Microarray technology
for identification and distinction of hantaviruses. J Med
Virol, 2004. 72(4): 646-55. |
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Schrader, J, Moyle, R, Bhalerao, R, Hertzberg,
M, Lundeberg, J, Nilsson, P, and Bhalerao, RP. Cambial meristem
dormancy in trees involves extensive remodelling of the
transcriptome. Plant J, 2004. 40(2): 173-87. |
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Sievertzon, M, Agaton, C, Nilsson, P, and
Lundeberg, J. Amplification of mRNA populations by a cDNA
tag strategy. Biotechniques, 2004. 36(2): 253-9. |
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