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Principal Investigator (PI): Prof
Joakim Lundeberg
Personnel: Anna Blomstergren (PhD), Stina
Boräng (PhD), Esther Edlund-Rose (PhD-student w. Peter
Nilsson ), Marcus Gry Björklund (PhD-student with Peter
Nilsson), Anna Gustafsson (PhD-student), Max Käller (PhD-student
w. Afshin Ahmadian), Emilie Hultin (PhD-student w. Afshin
Ahmadian), Cecilia Laurell (PhD-student with Peter Nilsson),
Johan Lindberg (PhD-student with Peter Nilsson), Erik Petterson
(PhD-student w. Fredrik Ponten), Maria Sievertzon (PhD-student),
Valtteri Wirta (PhD-student).
Description: The research program focuses
on development and application of technologies and bioinformatics
tools for identification of differentially expressed genes,
characterisation of single nucleotide polymorphisms, and identification
of genetic alterations in pathological conditions. The aims
with these technologies are to contribute to an improved understanding
of gene function in the studied biological systems. The developed
tools are currently used in a series of ongoing collaborations
covering both genetic analysis in metabolic and cancer disease,
and transcript analysis in poplar trees and neural stem cells.
The development of new technologies include Solid-phase representational
difference analysis (RDA), cDNA Microarrays, Transcriptome
amplification technologies, Single cell analysis in histological
tissues sections, Magnetic bead capture of nucleic acids,
Pyrosequencing of cDNA libraries and other genomic targets,
and SNP genotyping by apyrase mediated allele specific extension.
| Recent publications: |
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Agaton C., Sievertzon M., Holmberg
A., Larsson M., Unneberg P., Odeberg J., Uhlén M.,
and Lundeberg J. Gene expression analysis by signature pyrosequencing.
2002, Gene, 289, 31-39. |
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Andersson T., Unneberg P, Nilsson P, Odeberg
J, Quackenbush J and Lundeberg J., Monitoring of representational
difference analysis subtraction procedures by global microarrays,
2002, Biotechniques 32:1348-1358. |
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O' Meara D., Ahmadian A., Odeberg J. and
Lundeberg J. SNP typing by apyrase-mediated allele-specific
primer extension on DNA microarrays. 2002, Nucleic Acids
Res 30 (15) e75. |
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Savolainen P.,.Zhang Y., Luo J, Lundeberg
J, and Leitner T.Genetic evidence of an East Asian origin
of domestic dogs, Science, 2002, Nov 22;298(5598):1610-3. |
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Persson Å., Bäckvall H., Ponten
F., Uhlen M. and Lundeberg J. Single cell gene mutation
analysis using laser-assisted microdissection of tissue
sections. 2002 Meth Enzym, 356, 334-343. |
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Boräng S., Anderssson T., Thelin A.,
Odeberg J., and Lundeberg J. Vascular gene expression in
atherosclerotic plaque prone regions analysed by representational
difference analysis. 2004 Pathobiology, 71(2) 107-14. |
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Bäckvall H., Strömberg S., Asplund
A., Sivertsson Å., Lundeberg J., and Ponten F. Mutational
spectra of p53 clones adjacent to basal cell carcinoma and
squamous cell carcinoma, 2004, J. Invest Derm, in press. |
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Sivertzon M., Agaton C., Nilsson P. and Lundeberg
J. Transcriptome amplification from small tissue samples
2004, Biotechniques, in press. |
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