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Ines Ezcurra

From Glycoscience

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Principal Investigator (PI): Assoc. Prof. Ines Ezcurra

Office phone: +46 (0)8 5537 8388
E-mail:
Address: Please see the address page.

Curriculum vitae :: Research interests :: Selected publications :: All publications :: Current personnel :: Collaborators  :: Teaching

Research interests

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Our research deals with protein function and protein-protein interactions during secondary cell wall biosynthesis in trees. Using techniques of plant molecular biology, we are functionally characterizing proteins that are upregulated during secondary cell wall formation in the poplar xylem. Our efforts include establishment and transformation of P. trichocarpa Nisqually-1 cell cultures, as well as establishment of a platform for heterologous expression of poplar xylem proteins. We also study subcellular localization and protein co-localization by in vivo GFP imaging, as well as protein-protein interactions and protein phosphorylation.

Selected publications

  • Eklund DM, Ståldal S, Valsecchi I, Cierlik I, Eriksson C, Hiratsu K, Ohme-Takagi M, Sundström JF, Thelander M, Ezcurra I and Sundberg E (2010) The Arabidopsis thaliana STYLISH1 Protein Acts as a Transcriptional Activator Regulating Auxin Biosynthesis. Plant Cell 22, 349-363
  • Winzell A, Aspeborg H, Wang Y and Ezcurra I (2010) Conserved CA-rich motifs in gene promoters of PtxtMYB021-responsive secondary cell wall carbohydrate-active enzymes in Populus. Biochem Biophys Res Commun 394, 848-853
  • Guerriero G, Martin N, Golovko A, Sundström J, Rask L and Ezcurra I (2009) The RY/Sph element mediates transcriptional repression of maturation genes from late maturation to early seedling growth. New Phytologist 184, 552-565
  • Fugelstad J, Bouzenzana J, Djerbi S, Guerriero G, Ezcurra I, Teeri TT, Arvestad L, Bulone V (2009) Identification of the cellulose synthase genes from the Oomycete Saprolegnia monoica and effect of cellulose synthesis inhibitors on gene expression and enzyme activity. Fungal Genetics and Biology 46, 759-767
  • Hrmova M, Farkas V, Harvey AJ, Lahnstein J, Wischmann B, Kaewthai N, Ezcurra I, Teeri TT, Fincher GB (2009) Substrate specificity and catalytic mechanism of a xyloglucan xyloglucosyl transferase HvXET6 from barley (Hordeum vulgare L.). FEBS J 276, 437-56
  • Rajangam AS, Kumar M, Aspeborg H, Guerriero G, Arvestad L, Pansri P, Brown CJ, Hober S, Blomqvist K, Divne C, Ezcurra I, Mellerowicz E, Sundberg B, Bulone V, Teeri TT (2008). MAP20, a microtubule-associated protein in the secondary cell walls of Populus tremula L. x tremuloides Michx is a target of the cellulose synthesis inhibitor, 2,6-dichlorobenzonitrile. Plant Physiology 148, 1283-1294.
  • Wycliffe P, Sitbon F, Wernersson J, Ezcurra I, Ellerström M and Rask L (2005). Continuous expression in tobacco leaves of a Brassica napus PEND homologue blocks plastid differentiation and palisade cell development. Plant J. 44, 1-15.
  • Tang W, Kelley D, Ezcurra I, Cotter R, and McCormick S (2004). LeSTIG1, an extracellular binding partner for the pollen receptor kinases LePRK1 and LePRK2, promotes pollen tube growth in vitro. Plant J. 39, 343-353.
  • Tang W, Ezcurra I, Muschietti J, and McCormick S (2002). A cysteine-rich pollen allergen, LAT52, is a multimeric ligand for the pollen receptor kinase LePRK2. Plant Cell 14, 2277-2287.
  • Kim HU, Cotter R, Johnson S, Senda M, Dodds P, Kulikauskas R, Tang W, Ezcurra I, Herzmark P and McCormick S (2002). New pollen-expressed receptor kinases identified in tomato, maize and Arabidopsis: the tomato kinases show distinct but overlapping localization patterns during pollen tube growth. Plant Mol. Biol. 50, 1-16.
  • Ezcurra I, Wycliffe P, Nehlin L, Ellerstrom M, Rask L (2000). Transactivation of the Brassica napus napin promoter by ABI3 requires interaction of the conserved B2 and B3 domains of ABI3 with different cis-elements: B2 mediates activation through an ABRE, whereas B3 interacts with an RY/G-box. Plant J. 24, 57-66.

Other recommended reading

  • Teeri TT, Brumer H 3rd, Daniel G, Gatenholm P (2007) Biomimetic engineering of cellulose-based materials. Trends Biotechnol 25: 299-306
  • Ohlsson AB, Djerbi S, Winzell A, Bessueille L, Ståldal L, Li XG, Blomqvist K, Bulone V, Teeri TT, Berglund T (2006). Cell suspension cultures of Populus tremula x tremuloides exhibit a high level of cellulose synthase gene expression that coincides with increased in vitro cellulose synthase activity. Protoplasma 228: 221-229.
  • Aspeborg H, Schrader J, Coutinho PM, Stam M, Kallas A, Djerbi S, Nilsson P, Denman S, Amini B, Sterky F, Master E, Sandberg G, Mellerowicz E, Sundberg B, Henrissat B, Teeri TT. (2005). Carbohydrate-active enzymes involved in the secondary cell wall biogenesis in hybrid aspen. Plant Physiol. 137: 983-997.
  • Hertzberg M, Aspeborg H, Schrader J, Andersson A, Erlandsson R, Blomqvist K, Bhalerao R, Uhlen M, Teeri TT, Lundeberg J, Sundberg B, Nilsson P, Sandberg G. (2001). A transcriptional roadmap to wood formation. Proc. Natl. Acad. Sci. 98: 14732-14737.

Current personnel

  • Camilla Josefsson (Diploma student)
  • Prashanth Tamizhselvan (Diploma student)
  • Nomchit Kaewthai (PhD student co-supervised with Harry Brumer)
  • Christian Brown (PhD student co-supervised with Vincent Bulone)

Collaborators

  • Dr Ewa Mellerovicz, UPSC, Sweden
  • Prof Björn Sundberg, UPSC, Sweden
  • Prof Eva Sundberg, SLU Uppsala, Sweden

Teaching

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