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BB2420 - Glycobiology and Carbohydrate Technology/Syllabus

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The following is a preliminary list of lectures to provide a more detailed overview of the course content than that provided in the Course description (Kurs PM).

List of lectures

  • Course overview and Introduction to enzyme technology (Brumer)
    • Practical course details, overview of syllabus
    • Why Glycobiology & Carbohydrate Technology?
    • Overview of carbohydrate diversity and carbohydrate-active enzymes (CAZymes)
    • Examples of carbohydrate-based biotech applications
  • Carbohydrate structure: From monosaccharides to complex assemblies (Brumer)
    • The monsaccharides: structure, configuration, conformation, and linkage
    • Oligo- and polysaccharide structure and diversity - examples from Nature
  • Nucleotide sugar enzymology (Bulone)
    • Common sugars in plant and microbial cell walls
    • Structural glycans in animals
    • UDP-activated sugars as oligo- & polysaccharide precursors
    • UDP sugar interconversion: epimerization, oxidation, & decarboxylation
    • Introduction to glycosyl transferases
  • Enzymatic synthesis of glycosides/Enzymatic cleavage of glycosides I (Brumer)
    • Overview of carbohydrate-active enzymes (CAZymes)
    • Introduction to the CAZy database
    • Glycosyl transferases (GTs)
    • Glycoside hydrolases and transglycosidases (GHs)
    • Carbohydrate binding modules (CBMs)
  • Enzymatic cleavage of glycosides II (Brumer)
    • Case study: Hen egg white lysozyme
    • Importance of understanding enzyme mechanism in drug design
    • Polysaccharide lyases (PLs)
    • Carbohydrate esterases (CEs)
  • CAZyme structure/ function relationships (Divne)
    • Protein structure primer
    • CAZyme structural diversity
    • Structure-function relationships of CAZymes
  • CAZyme structure/ function relationships (Divne)
    • Molecular graphics lab with examples
  • Glycoproteins I (Bulone)
    • Glycoproteins versus proteoglycans
    • Functions of N- and O-glycans
    • Sites of biosynthesis of glycoproteins and the secretion pathway
    • Structure and biosynthesis of N-glycans
  • Glycoproteins II (Bulone)
    • An overview of cellular membrane trafficking and trafficking of N-glycoproteins
    • O-Linked glycosylation
      • Mucins and glycoproteins with mucin-like domains
      • Clusters of O-linked sugars in soluble and cell-surface glycoproteins
      • Biosynthesis of mucin-type glycans
      • An overview of the structure and biosynthesis of proteoglycans
      • Unusual types of O-glycosylations
      • Chemical glycation of proteins
  • Glycolipids (Bulone)
    • Overview of membrane structure and modes of anchoring of membrane-bound proteins
    • Structure and biosynthesis of glycolipids
      • Glycosyl-Phosphatidyl-Inositol (GPI) anchors
      • Glycosylated glycerophospholipids
      • Glycosphingolipids
    • Catabolism of glycosphingolipids and associated diseases
    • The structural role of glycosphingolipids in lipid rafts
  • Lignin-carbohydrate complexes (Henriksson)
    • Structure, biosynthesis, and breakdown
  • Applications of hydrolases in fiber processing (Henriksson)
    • Cellulases vs. cellulose
      • Targeted wood fiber degradation
      • Textile processing
    • Xylanases in pulp bleaching
    • Amylases in surface coating
  • Other enzymes in fiber processing & analysis (Brumer)
    • Harnessing plant genomics and biotechnology
    • Xyloglucanases for in vivo fiber modification
    • Xyloglucan endo-transglycosylases for ex vivo cellulose modification
  • Enzymes in food production (Brumer)
    • Starch processing
    • Glucose isomerase - the world's most important industrial enzyme
    • Phytic acid and the Enviropig(TM)
  • Enzymes in (bio)fuel production (Brumer)
    • Why biofuels?
    • Sugars in ethanol production: from Brazil to Sweden
      • sucrose bioethanol
      • starch bioethanol
      • lignocellulosic bioethanol
      • Biofuel crops
  • Medical applications I - Lectins (Brumer)
    • Roles in protein trafficking
    • Roles in innate immunity
    • Roles in therapeutic glycoprotein clearance
    • Toxicity and applications of plant lectins
    • Lectins as microbial toxins and bacterial adhesion molecules
  • Medical applications II (Brumer)
    • Influenza - hemagglutinins and neuraminidases
    • Fabry and Schindler diseases
    • Blood groups and blood group interconversion