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Since 1997, KTH has been part of a collaborative effort in
tree genomics. The three main parts of this consortium are
KTH Genome Center, Umeå Plant Science Center (UPSC)
and Wallenberg Wood Biotechnology Center. The activities at
the KTH Genome Center includes high-throughput sequencing
of cDNA clones, microarray production and sequencing of full-length
genes. To date, more than 100 000 ESTs (expressed sequence
tags) has been produced from 19 tissue-specific libraries
and a couple of hundred full-length inserts have been sequenced.
During recent years, thousands of microarrays have been produced,
each containing almost 14 000 spots representing unique transcripts.
At present, the poplar microarrays contain 25 000 spots, corresponding
to approximately 20 000 genes. We are now running a pilot
study for antibody proteomics (www.hpr.se),
where specific antibodies are being produced for studies of
wood development. Another promising effort is the development
of software for SNP discovery and a study of biodiversity
in aspen (Populus tremula).
| Key (own)
publications: |
| 1. |
Sterky et al. (2004) ”
A Populus EST resource for plant functional genomics”
Submitted PNAS |
| 2. |
Andersson et al. (2004) “A transcriptional
timetable of autumn senescence” Genome Biology 2004,
5(4):R24 |
| 3. |
Sterky et al. (2001) “Tree genomics
in Sweden: a national network of biological and technological
expertise” AgBiotechNet, 3:1-5. |
| 4. |
Hertzberg et al. (2001) ”A transcriptional
roadmap to wood formation” Proc Natl Acad Sci U S
A 98, 14732-14737 |
5. |
Hertzberg et al. (2001) ”cDNA microarray
analysis of small plant tissue samples using a cDNA tag
target amplification protocol” Plant J. 25(5):585-91 |
6. |
Sterky et al. (1998) “Gene discovery
in the wood-forming tissues of poplar: analysis of 5, 692
expressed sequence tags” Proc Natl Acad Sci U S A
95:13330-13335 |
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