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The ability to analyze very small amounts of material, as
well as being able to select exactly defined cell populations,
or even single cells, in heterogeneous material has been a
long sought goal for researchers in fields such as genetic
archaeology, forensics, prenatal diagnostics and tumor biology.
In the last couple of years developments in the fields of
microdissection and amplification has made this a reality.
Today there are several commercial laser-assisted systems
suitable for cell population and single cell dissection. We
have in collaboration with Uppsala University (Dr Fredrik
Ponten) developed standardized protocols to analyse genomic
DNA in single cells obtained from tissue sections. In addition
we have have developed strategies to subtract and amplify
the RNA content from minute amounts of sample that is well
suited for microarray analysis.
| Key (own)
publications: |
| 1. |
Ponten F, Williams C, Ling G,
Ahmadian A, Nister M, Lundeberg J, Ponten J, Uhlen M. Genomic
analysis of single cells from human basal cell cancer using
laser-assisted capture microscopy. Mutat Res. 1997 Sep;382(1-2):45-55. |
| 2. |
Williams C., Pontén F., Ahmadian A.,
Ren Z-P., Ling G., Rollman O., Ljung A., Nicholaas G.J.J.,
Uhlén M., Lundeberg J. and Pontén J. Clones
of normal keratinocytes and a variety of simultaneously
present epidermal neoplastic lesions contain a multitude
of p53 gene mutations in a Xeroderma Pigmentosum patient.
1998 Cancer Res 58, 2449-2455. |
| 3. |
Odeberg J., Wood T., Blücher Å.,
Raphter J., Norstedt G., and Lundeberg J. A modified cDNA
RDA protocol on solid-phase technology suited for analysis
of gene expression in small tissue samples. 2000, Biomol
Engineering, 17(1), 1-9. |
| 4. |
Persson Å.E., Ling G., Williams C.,
Bäckvall H., Pontén J., Pontén F. and
Lundeberg J. Analysis of p53 mutations in single cells obtained
from histological tissue sections. 2000, Anal Bichem, 287,
25-31. |
5. |
Hertzberg M., Sivertzon M., Aspeborg H.,
Nilsson P., Sandberg G., and Lundeberg J. cDNA microarray
analysis of small plant tissue samples using a cDNA tag
target amplification protocol. 2001, Plant J, 25(5), 585-591. |
6. |
Hertzberg M., Aspeborg H., Schrader J., Anderson
A., Blomqvist K., Bhalereo R., Rahman D., Marchant A., Melerowicz
E.J., Bennett M., Uhlén M., Teeri T., Lundeberg J.,
Sundberg B., Nilsson P., Sandberg G. A transcriptional roadmap
to wood formation, 2001, Proc Nation Acad Science, 98, 14732-14737. |
7. |
Ling Gao, Persson Åsa, Berne Berit,
Lundeberg Joakim, Ponten Fredrik: Persistent p53 mutations
in single cells from normal human skin. American Journal
of Pathology 159: 1247-1253, 2001. |
8. |
Åsa E. Persson Desiree Wiegleb Edström
Helena Bäckvall, Joakim Lundeberg, Fredrik Pontén,
Anne-Marie Ros and Cecilia Williams: The Mutagenic Effect
of UVA1 in Human Skin: An analysis of the p53 gene in single
keratinocytes. 2002, Photoderm, Photoimm and Photomed, in
press. |
9. |
Andersson T., Unneberg P, Nilsson P, Odeberg
J, Quackenbush J and Lundeberg J., Monitoring of representational
difference analysis subtraction procedures by global microarrays,
2002, Biotechniques, 32:1348-1358. |
10. |
Sievertzon M, Agaton C, Nilsson P, Lundeberg
J Amplification of mRNA populations by a cDNA tag strategy.
Biotechniques. 2004 Feb;36(2):253-9. |
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