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In 1988, we showed for the
first time that the biotin-streptavidin system could be
used for efficient solid phase sequencing (1). Magnetic
beads were developed to facilitate the solid phase handling
(2,3,4). A new principle for DNA diagnostics was also
developed called DIANA (Detection of Immobilized Amplified
Nucleic Acids) based on the streptavidin system (5,6).
The sequencing approach was used for analysis of clinical
samples, such as patients with HIV (7) and HCV (8) infections
and for forensic analysis (9,10). Extensive evolutionary
studies was also performed to study the evolution of bacteria
(9) and domestic dogs (12) as well as the epidemiology
of HIV infections in patients (8). |
| Key (own)
publications: |
| 1. |
Stahl, Hultman, Olsson, Moks
& Uhlen Solid phase DNA sequencing using the biotin-avidin
system. Nucleic Acids Res 16, 3025-3038 (1988). |
| 2. |
Hultman, Stahl, Hornes & Uhlen Direct
solid phase sequencing of genomic and plasmid DNA using
magnetic beads as solid support. Nucleic Acids Res 17, 4937-4946
(1989). |
| 3. |
Uhlen Magnetic separation of DNA. Nature
340, 733-734 (1989). |
| 4. |
Hultman, Murby, Stahl, Hornes & Uhlen
Solid phase in vitro mutagenesis using plasmid DNA template.
Nucleic Acids Res 18, 5107-5112 (1990). |
5. |
Wahlberg, Lundeberg, Hultman & Uhlen
General colorimetric method for DNA diagnostics allowing
direct solid-phase genomic sequencing of the positive samples.
Proc Natl Acad Sci U S A 87, 6569-6573 (1990). |
6. |
Lundeberg J., Wahlberg J. and Uhlén
M., Rapid colorimetric quantification of PCR-amplified DNA.
BioTechniques 10 (1). |
7. |
Scarlatti et al. Comparison of variable region
3 sequences of human immunodeficiency virus type 1 from
infected children with the RNA and DNA sequences of the
virus populations of their mothers. Proc Natl Acad Sci U
S A 90, 1721-1725 (1993). |
8. |
Odeberg J., Yun Z., Sönnerborg A., Uhlén
M. and Lundeberg J. Dynamic changes of heterogenous hepatitis
C virus populations. 1995, J. Clin. Microbiol, 33, 1870-1874.
9. Albert, Wahlberg and Uhlen Forensic evidence by DNA sequencing
Nature 361:595-596 (1993) |
9. |
Leitner, Escanilla, Franzen, Uhlen &
Albert Accurate reconstruction of a known HIV-1 transmission
history by phylogenetic tree analysis. Proc Natl Acad Sci
U S A 93, 10864-10869 (1996). |
10. |
Pettersson et al. Evolutionary relationships
among members of the genus Chlamydia based on 16S ribosomal
DNA analysis. J Bacteriol 179, 4195-4205 (1997). |
11. |
Savolainen P., Rosén B., Holmberg
A., Leitner T., Uhlén M., and Lundeberg J.; Analysis
of domestic dog mitochondrial DNA for forensic use. 1997,
J. Foren. Sci., 591-598 |
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