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Several automated systems for sample handling
has been developed (1,2). The sample preparation is based
on an “in-house” designed robotic work station
allowing magnetic separation and temperature-based hybridization/annealing.
These systems have been used for applications involving sequencing
(1,2,3), cell capture (4,5) and microbial/viral diagnostics
(6,7). Recently, a method to regenerate the biotin-streptavidin
interaction has been developed to allow re-use of streptavidin
magnetic beads at least six times without loss of capacity
or yield (6). Thus, cost-effective, high-throughput schemes
for genomics and proteomics can be envisioned, including solid
phase cloning, sequencing clean-up and sample preparation
for cDNA arrays for transcript profiling. A commercial instrument
(Magnatrix1200) based on the “in-house” designed
robot has recently been developed in collaboration with two
companies (Japan and Sweden).
| Key (own)
publications: |
| 1. |
Uhlén M., Hultman T.,
Wahlberg J., Lundeberg J., Bergh S., Petersson, Holmberg
A., Ståhl S. and Moks T. Semi-automated solid-phase
DNA sequencing. Trends Biotechnol 10, 52-55 (1992). |
| 2. |
Wahlberg, Holmberg, Bergh, Hultman &
Uhlen. Automated magnetic preparation of DNA templates for
solid phase sequencing. Electrophoresis 13, 547-551 (1992). |
| 3. |
Blomstergren A., O’ Meara D., Lukacs
M., Uhlén M., and Lundeberg J., Cooperative oligonucleotides
in enrichment of cycle sequencing products. 2000, BioTechniques,
29(2), 352-358). |
| 4. |
Seesod N., Lundeberg J., Hedrum A., Åslund
L., Holder A., Thaithong S. and Uhlén M. Immunomagnetic
purification to facilitate DNA diagnosis of Plasmodium falciparum.
J. Clin Microbiol 31(10), 1993, 2715-2719 |
5. |
Stark M., Reizenstein E., Uhlén M.,
and Lundeberg J. Immunomagnetic separation and solid-phase
detection of Bordetella pertussis. 1996, J. Clin. Microbiol.
34, 778-784 |
6. |
Olsvik et al. Magnetic separation techniques
in diagnostic microbiology. Clin Microbiol Rev 7, 43-54
(1994). |
7. |
O’ Meara D., Yun Z., Sönnerborg
A., and Lundeberg J. Cooperative oligonucleotides mediating
in the direct capture of hepatitis C virus RNA from serum.
1998, J. Clin Microbiol. 36(9), 2454-9 |
8. |
Holmberg et al (2002) “Regeneration
of biotin-streptavidin magnetic beads”, manuscript |
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