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		Sven-Olof Enfors, PhD Prof. emeritus of biochemical technology Department of Bioprocess Technology KTH School of Biotechnology Royal Institute of Technology (KTH) Roslagstullsbacken 21 S-106 91 Stockholm phone. + 46-8-5537 8302 fax: + 46-8-5537 8323 email: enfors@biotech.kth.se

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Some theses for downloading: Rozkov Aleksei: Control of proteolysis of recombinant proteins in Escherichia coli (pdf-file) PhD thesis, 2001. Jahic Mehmedalija: Process techniques for production of recombinant proteins with Pichia pastoris (pdf-file) PhD thesis, 2003. Charoenrat Thepanya: Process design for production of Thai rosewood ß-glucosidase with Pichia pastoris (pdf-file) Licential thesis, 2005. Svensson Marie: The temperature limited fed-batch techniques for control of Escherichia coli cultures (pdf-file) PhD thesis 2006. Heléne Sundström: Process analytical techniques for fermentation processes PhD thesis 2007 Yanling Liu: Electric DNA chip arrays for determination of pathogenic microorganisms (pdf-file) PhD thesis 2008

Teaching material Enfors - Häggström: Bioprocess Technology - Fundamentals and Applications, KTH 2000. A textbook for introduction of the theory and practice of biotechnical processes (350 p.) Food Microbiology (89 pages. 3.7 MB) ------------------------------------------------------------------------------------------------- For the EFB BEC course, Brac, September 14-19, 2008: Lecture slides: Basic biological concepts Integrated Pichia technology Simlite: A MATLAB toolbox for interactive simulation of bioprocesses. -------------------------------------------------------------------------------------------------- PilotTools: A MATLAB toolbox for fermentation process data handling and plotting --------------------------------------------------------------------------------------------------

BetaGalSim: MATLAB based program for training in fed-batch fermentation and extraction of the intracellular protein ß-galactosidase in E. coli. The task is to produce minimum 50 000 kU ß-galactosidase with a specific activity of minimum 400 kU/g protein. To accomplish this in the 250L fermenter fed-batch technique must be used. The fermentation is controlled by the glycerol feed rate, the stirrer rate and the aeration rate. The process design is started with simulated determination of KLa using the dynamic method. Based on this, the maximum feed rate and the minimum cell concentration required to consume this feed (start of the fed batch) is calculated. From the required cell mass, the initial glycerol concentration for the batch is calculated. Then the process simulations is started. When sufficient product has been obtained, the cells are harvested by centrifugation and disintegrated by high-pressure homogenization. The cell homogenate is then transferred to the extraction, where a suitable aqueous two-phase system (PEG-potassium phosphate) is selected from the phase diagramme: Downwnload the BetaGalSim manual Download the BetaGalSim programe package (requires MATLAB v5.2 or later): Mac version Windows version

Links

• Department of Bioprocess Technology homepage
• School of Biotechnology homepage

Updated: 2009-07-21