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Pål Nyrén, professor i biokemi
Short biography
1999 Professor in Biochemistry, KTH,
Stockholm
1997 Founder of the company Biotage AB (former Pyrosequencing AB)
1988 Associate professor (Docent) Biochemistry University of
Stockholm
1985-86 Postdoc at LMB, MRC, Cambridge, G.B. with prof John Walker
1985 Ph.D. (Tekn. Doktor) Biochemistry, University of Stockholm
1981 M.Sc. (Civ. ing.) Chemical Engineering, KTH, Stockholm
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Research in Applied Enzymology |
See the link to the research database at KTH:
Technology development in the field of DNA diagnostics |
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Key Publications |
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Nyrén, P. (2007) The history of Pyrosequencing. Methods Mol. Biol.
373, 1-14. |
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Akhras, M., Unemo, M., Thiyagarajan, S., Nyrén, P., Davis, R. Fire, A.
and Pourmand, N. (2007) Connector Inversion Probe technology; a
Powerful One-Primer Multiplex DNA Amplification System for numerous
scientific applications. PloS ONE Sept. Iss. 9, e915. |
P. Sakthivel, X.B. Wang, B. Gharizadeh, R.Giscombe, R. Pirskanen, P.
Nyrén, and A.K.
Lefvert (2007) Single-nucleotide polymorphisms in the B7H3 gene are
not associated with human autoimmune myasthenia gravis. Journal
of Genetics 85, 3, 217-220. |
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Gharizadeh, B., Akhras, M., Nourizad, N., Ghaderi, M., Yasuda, K.,
Nyrén, P., and Pourmand, N. (2006) Methodological improvements of
Pyrosequencing technology. J. Biotechnol.124 (3), 504-11. |
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Gharizadeh, B.,Zheng, B., Akhras, M., Ghaderi, M., Jejelowa, O.,
Strander, B., Nyrén, P., Wallin, K-L., and Pourmand, N. (2006)
Sentinel-base DNA genotyping using multiple sequencing primers for
high-risk human papillomaviruses. Mol. Cell. Probes, 20(3-4),
230-8 |
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S. Svantesson, P.O. Westermark, J. Hellgren Kotaleski, B. Gharizadeh,
A. Lansner and P. Nyrén (2004) A mathematical model of the
Pyrosequencing reaction system. Biophysical Chemistry 110,
129-145. |
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B. Gharizadeh, M. Ghaderi, D. Donnelly, K-L. Wallin, and P. Nyrén (2003)
Multiple-primer DNA sequencing method. Electrophoresis, 24,
1145-1151. |
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T.Nordström, A. Alderborn, and P. Nyrén (2002) Method for
one-step preparation of double-stranded DNA template applicable for
use with Pyrosequencing technology. J. Biochem. Biophys. Methods
52, 71-82. |
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B. Gharizadeh, T. Nordström, A. Ahmadian, M. Ronaghi and P. Nyrén (2001)
Long read pyrosequencing using pure
2’-deoxyadenosine-5’-O’-(1-thiotriphosphate) Sp-isomer. Anal.
Biochem. 301, 82-90. |
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B. Gharizadeh, M. Kalantari, C. Garcia, B. Johansson, and P. Nyrén (2001)
Typing of human papillomavirus (HPV) by pyrosequencing. Laboratory
Investigation 81, 673-679. |
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M. Ronaghi, M. Uhlén, and P. Nyrén (1998) A sequencing method
based on real-time pyrophosphate. Science 281, 363-365. |
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P. Nyrén, B.F. Nore and Å.Strid (1990) Proton pumping
DCCD-sensitive inorganic pyrophosphatase from Rhodospirillum rubrum:
Purification, characterization and reconstitution. Biochemistry
30, 2883-2887. |
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P. Nyrén (1987) Enzymatic method for continuous monitoring of
DNA-polymerase activity. Anal. Biochem. 167, 235-238. |
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M.J. Runswick, S.J. Powell, P. Nyrén and J.E. Walker (1987)
Sequence of the bovine mitochondrial phosphate carrier protein:
Structual relationship to ADP/ATP translocase and the brown fat
mitochondria uncoupling protein. EMBO J. 6, 1367-1373. |
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P. Nyrén, B.F. Nore and M. Baltscheffsky (1986) Inorganic
pyrophosphate synthesis after a short light flash in chromatophores
from Rhodospirillum rubrum. Photobiochem. Photobiophys. 11,
189-196. |
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P. Nyrén and M. Baltscheffsky (1983) Inorganic pyrophos-phate
driven ATP-synthesis in liposomes containing membrane-bound inorganic
pyrophosphatase and FoF1 complex from Rhodospirillum rubrum. FEBS
Lett. 155, 125-130. |
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