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Publications from the
Biocatalysis Group
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For older publications, please see here:
1992-2002 |
Ph.D. theses |
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2012
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Maria S. Humble,
Karim E. Cassimjee, Maria Håkansson, Yengo R. Kimbung, Björn
Walse, Vahak Abedi, Hans-Jürgen Federsel, Per Berglund*, Derek
T. Logan*. Crystal structures of the Chromobacterium
violaceum
w-transaminase
reveal major structural rearrangements upon binding of coenzyme
PLP. FEBS J. 2012, 279,
779-792.
doi:10.1111/j.1742-4658.2012.08468.x |
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Syrén P-O, Hendil-Forssell P, Aumailley L, Besenmatter W, Gounine
F, Svendsen A, Martinelle M and Hult K (2012) Esterases with an
introduced amidase-like hydrogen bond in transition state have
increased amidase specificity. ChemBioChem 13,
645-648. |
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Maria
Svedendahl Humble, Karim Engelmark Cassimjee, Vahak Abedi, Hans-Jürgen
Federsel and Per Berglund*. Key Amino Acid Residues for
Reversed or Improved Enantiospecificity of an
ω-Transaminase.
ChemCatChem 2012, in press.
doi:10.1002/cctc.201100487 |
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2011
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Maria Svedendahl Humble, Per Berglund.
Biocatalytic Promiscuity. Eur. J. Org. Chem. 2011, 2,
3391-3401. DOI:
10.1002/ejoc.201001664 |
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Karim Engelmark
Cassimjee, Maria Svedendahl Humble, Valentina Miceli, Carla Granados
Colomina, Per Berglund.
Active
site quantification of an
ω-transaminase
by performing a half transamination reaction. ACS Catal.
2011, 1, 1051–1055.
DOI:
10.1021/cs200315h |
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Karim Engelmark
Cassimjee, Robert Kourist, Diana Lindberg, Marianne Wittrup
Larsen, Nguyen Hong Thanh, Mikael Widersten, Uwe T. Bornscheuer,
Per Berglund.
One-step enzyme extraction and immobilization for Biocatalysis
applications. Biotechnol. J. 2011, 6,
463-469.
DOI: 10.1002/biot.201000357 |
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Pornrapee
Vongvilai, Mats Linder, Morakot Sakulsombat, Maria Svedendahl,
Per Berglund, Tore Brinck, Olof Ramström.
Racemase Activity of B. cepacia Lipase Leads to
Dual-Function Asymmetric Dynamic Kinetic Resolution of
a-Aminonitriles.
Angew. Chem. Int. Ed. 2011, 50, 6592-6595.
DOI: 10.1002/anie.201007373.
Angew. Chem. 2011, 123, 6722-6725
DOI: 10.1002/ange.201007373. |
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Karim Engelmark
Cassimjee, Maria Svedendahl Humble, Valentina Miceli, Carla
Granados Colomina, Per Berglund.
Active site quantification of an
w-transaminase
by performing a half transamination reaction. ACS Catal.
2011, 1, 1051–1055.
DOI: 10.1021/cs200315h |
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Engström K Vallin M, Syrén P-O, Hult K and Bäckvall J-E (2011)
Mutated variant of Candida antarctica lipase B in (S)-selective
dynamic kinetic resolution of secondary alcohols. Org Biomol
Chem 9, 81-82. |
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Syrén P-O and Hult K (2011) Amidases have a hydrogen bond that
facilitates nitrogen inversion but esterases have not.
CatChemCat 5, 853-860. |
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Eriksson M, Hult K, Malmström E, Johansson M, Trey S M and
Martinelle M (2011) One-pot enzymatic polycondensation to
telechelic methacrylate-functional oligoesters used for film
formation. Polym Chem 2, 714-719. |
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Takwa M, Wittrup Larsen M, Hult K and Martinelle M (2011).
Rational redesign of Candida antarctica lipase B for the ring
opening polymerization of D,D-lactide. ChemComm 47,
7392–7394. |
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2010
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Karim Engelmark Cassimjee, Cecilia Branneby, Vahak
Abedi, Andrew Wells and Per Berglund. Transaminations with isopropyl
amine: equilibrium displacement with yeast alcohol dehydrogenase
coupled to in situ cofactor regeneration. Chem. Commun.
2010, 46, 5569-5571.
http://dx.doi.org/10.1039/c0cc00050g |
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Maria Svedendahl, Cecilia Branneby, Lina Lindberg
and Per Berglund. Reversed Enantiopreference of an
w-Transaminase by a
Single-Point Mutation. ChemCatChem 2010, 2,
976-980.
http://dx.doi.org/10.1002/cctc.201000107 |
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Marton Z, Léonard-Nevers V, Syrén P-O, Bauer C,
Lamare S, Hult K, Tranc V and Graber M. Mutations in the
stereospecificity pocket and at the entrance of the active site of
Candida antarctica lipase B enhancing enzyme
enantioselectivity. J Mol Cat B
2010, 65,
11-17. |
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Hedfors C, Hult K and Martinelle M.
Lipase chemoselectivity towards alcohol and thiol
acyl acceptors in a transacylation reaction.
J Mol Cat B (in press). |
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Fransson L, Laurell A, Widyan K, Wingstrand E,
Hult K and Moberg C. Minor enantiomer recycling—Effect of two
reinforcing catalysts on product yield and enantiomeric excess.
ChemCatChem 2010,
2,
683-693. |
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Syrén P-O, Lindgren E, Hoeffken HW, Branneby C,
Maurer S, Hauer B and Hult K. Increased activity of enzymatic
transacylation of acrylates through rational design of lipases.
J Mol Cat B
2010,
65, 3–10. |
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Syrén P-O and Hult K. Substrate conformations set
the rate of enzymatic acrylation by lipases. ChemBioChem
2010,
11,
802-810. |
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Larsen MW, Zielinska DF, Martinelle M, Hidalgo A,
Jensen LJ, Bornscheuer UT and Hult K. Suppression of water as a
nucleophile in Candida antarctica lipase B catalysis.
ChemBioChem 2010,
11,
796-801. |
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Gardossi L, Poulsen PB, Ballesteros A, Hult K,.
Švedas VK, Vasić-Rački Ð, Carrea G, Magnusson A, Schmid A,
Wohlgemuth R and Halling PJ. Guidelines for reporting of
biocatalytic reactions. Trends Biotechnol
2010,
28, 171-180. |
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Vallin M, Syrén P-O and Hult K. Mutant
lipase-catalyzed kinetic resolution of bulky phenyl alkyl
sec-alcohols: A thermodynamic analysis of enantioselectivity.
ChemBioChem 2010,
11,
411-416. |
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2009
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Yan Xiao, Mohamad Takwa, Karl Hult, Cor E. Koning, Andreas Heise, Mats
Martinelle. Systematic comparison of hydroxyethyl acrylate (HEA) and
hydroxyethyl methacrylate (HEMA) as initiators in enzymatic
ring-opening polymerization. Macromol. Biosci. 2009,
9 , 713-720. DOI:
10.1002/mabi.200800290 |
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Maria Svedendahl, Biljana Jovanović, Linda Fransson, Per Berglund.
Suppressed Native Hydrolytic Activity of a Lipase to Reveal
Promiscuous Michael Addition Activity in Water. ChemCatChem
2009, 1, 252-258.
DOI:
10.1002/cctc.200900041 |
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Eriksson M, Fogelstroem L, Hult K, Malmstroem E,
Johansson M, Trey S, and Martinelle M. Enzymatic one-pot route to
telechelic polypentadecalactone epoxide: Synthesis, UV curing, and
characterization. Biomacromolecules
2009,
10, 3108-3113.
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Wingstrand E; Laurell A; Fransson L; Hult K;
Moberg C. Minor enantiomer recycling: Metal catalyst, organocatalyst
and biocatalyst working in concert. Chem Eur J
2009,
15, 12107-12113. |
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Léonard-Neversa V, Marton Z, Lamare S, Hult K,
Graber M. Understanding water effect on Candida antarctica
lipase B activity and enantioselectivity towards secondary alcohols.
J Mol Cat B 2009, 59, 90-95. |
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Veld MAJ, Fransson L, Palmans ARA, Meijer EW and
Hult K Lactone size dependent reactivity in Candida antarctica
lipase B: a molecular dynamics and docking study. ChemBioChem
2009, 10, 1330-1334. |
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2008
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Mohamad Takwa, Yan Xiao, Neil Simpson, Eva Malmström, Karl Hult, Cor
E. Koning, Andreas Heise, Mats Martinelle. Lipase catalyzed HEMA
initiated ring-opening polymerization: In-situ formation of mixed
polyester methacrylates by transesterification. Biomacromolecules
2008, 9, 704–710. |
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Cristian Vaida, Mohamad Takwa, Mats Martinelle, Karl Hult, Helmut Keul,
Martin Möller. Acyloxy-caprolactones: Synthesis, ring-opening
polymerization vs. rearrangement by means of chemical and enzymatic
catalysis. Macromol. Symp. 2008, 272, 28–38. |
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Neil Simpson, Mohamad Takwa, Eva Malmström, Karl Hult, Mats
Martinelle, Mats Johansson. Thiol Functionalised
Poly(?-pentadecalactone) Macro-monomer for Semi-crystalline Polymer
Networks. Macromolecules 2008, 41, 3613-3618. |
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Mohamad Takwa, Karl Hult and Mats Martinelle.One-step, solvent free
enzymatic route to functionalized poly-pentadecalactone
macromonomers. Macromolecules 2008, 41,
5230-5236. |
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Maria Svedendahl, Peter Carlqvist, Cecilia Branneby, Olof Allnér,
Anton Frise, Karl Hult, Per Berglund, Tore Brinck. Direct
Epoxidation in Candida antarctica lipase B Studied by Experiments
and Theory. ChemBioChem 2008, 9, 2443-2451.
http://dx.doi.org/10.1002/cbic.200800318 |
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Karim Engelmark Cassimjee, Martin Trummer, Cecilia Branneby and Per
Berglund. Silica-Immobilized His6-tagged enzyme: Alanine racemase in
hydrophobic solvent. Biotechnol. Bioeng. 2008, 99,
712-716.
http://dx.doi.org/10.1002/bit.21587 |
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Per Berglund. Book Review: Asymmetric Organic Synthesis with Enzymes,
Edited by Vicente Gotor, Ignacio Alfonso and Eduardo Garcia-Urdiales.
Angew. Chem. Int. Ed. 2008, 47, 6514-6515;
Angew. Chem. 2008, 120, 6614 – 6615.
http://dx.doi.org/10.1002/anie.200885614;
http://dx.doi.org/10.1002/ange.200885614 |
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Kourist R, Bartsch S, Fransson L, Hult K, Bornscheuer UT (2008)
Understanding promiscuous amidase activity of an esterase from
Bacillus subtilis. ChemBioChem 9: 67-69. |
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2007
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Karl Hult and Per Berglund. Enzyme promiscuity: mechanism and
applications. Trends Biotechnol. 2007, 25,
231-238.
http://dx.doi.org/10.1016/j.tibtech.2007.03.002 |
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Veld
MAJ, Hult K, Palmans ARA and Meijer EW (2007) Fast DKR of amines
using isopropyl 2-methoxyacetate as acyl donor. Eur J Org Chem (In
press) |
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Graber
M, Irague R, Rosenfeld E, Lamare S, Franson L and Hult K (2007)
Solvent as a competitive inhibitor for Candida antarctica
lipase B. Bio Bio Acta 1774: 1052-1057. |
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Marzorati M, Hult K, Riva S and Danielia B (2007) Incorporation of
primary amines into a polyester chain by a combination of chemical
and lipase-catalyzed -caprolactone ring-opening processes. Adv
Synth Catal 349: 1963-1968. |
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Hamberg A, Lundgren S, Wingstrand E, Moberg C and Hult K (2007)
High-throughput synthesis and analysis of acylated cyanohydrins.
Chem Eur J 13: 4334-4341. |
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Léonard V, Fransson L, Lamare S, Hult K and Graber M (2007) A water
molecule in the stereospecificity pocket of Candida antarctica
lipase B enhances the enantioselectivity towards 2-pentanol.
ChemBioChem 8: 662-667. |
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Heinze
B, Kourist R, Fransson L, Hult K and Bornscheuer UT (2007) Highly
enantioselective kinetic resolution of two tertiary alcohols using
mutants of an esterase from Bacillus subtilis. Prot Eng,
Design & Selection 20: 125–131. |
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2006
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Cammenberg M, Hult K and Park S (2006) Molecular basis for the
enhanced lipase-catalyzed N-acylation of 1-phenylethanamine
with methoxyacetate. ChemBioChem 7: 1745-1749. |
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Takwa
M, Simpson N, Malmström E, Hult K and Martinelle M (2006) One-pot
difunctionalization of poly(w-pentadecalactone)
with thiol-thiol or thiol-acrylate groups, catalyzed by Candida
antarctica lipase B. Macromol Rapid Commun 27: 1932-1936. |
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Per Berglund. Book Review: Hydrolases in Organic Synthesis: Regio- and
Stereoselective Biotransformations By Uwe T. Bornscheuer and Romas
J. Kazlauskas. ChemBioChem 2006, 7, 1280.
http://dx.doi.org/10.1002/cbic.200600269 |
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Hamberg A, Kempka M, Sjödahl J, Roeraade J and Hult
K. C-terminal ladder sequencing of peptides
using an alternative nucleophile in carboxypeptidase Y digests.
Anal Biochem 2006, 357, 167-172. |
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Hamberg A, Lundgren S, Penhoat M, Moberg C and Hult
K. High-throughput enzymatic method for enantiomeric excess
determination of O-acetylated cyanohydrins.
J Am Chem Soc
2006,
128, 2234-2235. |
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2005
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Svedendahl M, Hult K and Berglund P. Fast C-C bond
formation by a promiscuous lipase. J Am
Chem Soc
2005, 127,
17988-17989. |
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Hedin E M K, Højrup P, Patkar S A, Vind J, Svendsen
A and Hult K. Implications of surface charge and curvature for the
binding orientation of Thermomyces lanuginosus lipase on
negatively charged or zwitterionic phospholipid vesicles as studied by
ESR spectroscopy. Biochemistry
2005, 44,
16658-16671. |
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Magnusson AO, Takwa M, Hamberg A, and Hult K. An
S-selective lipase was created by rational redesign and the
enantioselectivity increased with temperature Angew Chem Int Edit
2005, 44, 4582-4585. |
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Vallikivi I, Fransson L, Hult K, Järving I, Pehk T,
Samel N, Tougu V, Villo L and Parve O. The modelling and kinetic
investigation of the lipase-catalysed acetylation of stereoisomeric
prostaglandins. J Mol Cat B,
2005,
35, 62-69. |
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Bernhardt P, Hult K and Kazlauskas R J. Molecular
basis of perhydrolase activity in serine hydrolases. Angew Chem Int
Edit 2005, 44, 2742-2746. |
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Magnusson AO, Rotticci-Mulder JC, Santagostino A
and Hult K. Creating space for large secondary alcohols by rational
redesign of Candida antarctica lipase B. ChemBioChem
2005, 6, 1051-1056. |
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Hedfors C, Östmark E, Malmström
E, Hult K and Martinelle M.
Thiol end-functionalization of poly(ε-caprolactone),
catalyzed by Candida antarctica lipase B. Macromolecules
2005, 38, 647-649. |
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Park S, Morley K, Horsman G, Holmquist M, Hult K
and Kazlauskas R. Focusing mutations into the substrate-binding site
of Pseudomonas fluorescens esterase increases the
enantioselectivity more effectively than distant mutations.
Chemistry & Biology
2005, 12,
45-54. |
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Gustavsson M, Persson PV, Iversen T,
Martinelle M, Hult K, Teeri T and Brumer H. Modification of cellulose
fiber surfaces by use of a lipase and a xyloglucan
endotransglycosylase. Biomacromolecules 2005, 6,
196-203. |
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Rong Liu, Per Berglund,
Hans-Erik Högberg. Preparation of the Four Stereoisomers of
3-Bromo-2-Butanol or their Acetates via Lipase-Catalysed Resolutions
of the Racemates from dl- or meso-2,3-Butanediol. Tetrahedron:
Asymmetry 2005, 16, 2607-2611. |
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Peter Carlqvist, Maria Svedendahl, Cecilia Branneby, Karl Hult, Tore Brinck, Per
Berglund.
Exploring the Active-Site of a Rationally Redesigned Lipase for Catalysis of
Michael-Type Additions.
ChemBioChem 2005, 6, 331-336. |
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Per Berglund, Seongsoon Park. Strategies for Altering Enzyme Reaction
Specificity for Applied Biocatalysis. Curr. Org. Chem. 2005,
9, 325-336. |
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Per Berglund. Enzymes Engineered for New Reactions - Novel Catalysts
for Organic Synthesis. Chapter 9 in Enzyme Technology, Pandey,
A.; Webb, C.; Soccol, C. R.; Larroche, C. (eds), AsiaTech Publishers,
Inc: New Delhi, 2005. pp. 175-188. ISBN 81-87680-12-1. |
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2004
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Cecilia Branneby, Peter Carlqvist, Karl Hult, Tore Brinck, Per
Berglund. Aldol Additions with Mutant Lipase: Analysis by Experiments
and Theoretical Calculations. J. Mol. Catal. B: Enzym. 2004,
31, 123-128. |
Hedin E M K, Hult K, Mouritsen OG and Højrup P, (2004). Low
microwave-amplitude ESR spectroscopy: measuring spin relaxation
interactions of moderately immobilized spin labels in proteins.
J Biochem Biophys Methods, 60: 117-138. |
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Viklund F and Hult K (2004). Enzymatic synthesis of
surfactants based on polyethylene glycol and stearic or 12-hydroxystearic acid.
J.
Mol. Catal. B: Enzym. 27:51-53. |
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Gustavsson MT, Persson PV, Iversen T, Hult K, Martinelle M (2004).
Polyester coating of cellulose fiber surfaces catalyzed by a
cellulose-binding module - Candida antarctica lipase B fusion
protein. Biomacromolecules, 5: 106-112. |
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2003
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Viklund F, Alander J and Hult K (2003).
Antioxidative properties and enzymatic synthesis of ascorbyl fatty acid esters.
J Am Oil Chemists' Soc, 80: 795-799. |
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Carlqvist P, Eklund R, Hult K and Brinck
T (2003). Rational design of a lipase to accommodate catalysis of
Baeyer-Villiger oxidation with hydrogen peroxide. J Mol Model, 9:
164-171. |
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Park S, Viklund F, Hult K and Kazlauskas
RJ (2003). Ionic liquids create new opportunities for nonaqueous biocatalysis
with polar substrates: Acylation of glucose and ascorbic acid.
ACS Symposium Series 856: 225-238. |
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Park S, Viklund F, Hult K and Kazlauskas RJ (2003). Vacuum-driven
lipase-catalyzed direct condensation of L‑ascorbic acid and fatty
acids in ionic liquids: Synthesis of a natural surface active
antioxidant. Green Chemistry, 5: 715-719. |
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Hult K and Berglund P (2003). Engineered enzymes for
improved organic synthesis. Curr. Opin. Biotechnol. 14: 395-400. |
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Lehtiö J, Sugiyama J, Gustavsson M, Fransson L, Linder M,
Teeri TT (2003). The binding specificity and affinity determinants of family 1
and family 3 cellulose binding modules. Proc. Natl. Acad.
Sci. U.S.A. 100: 484-489. |
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Jahic M, Gustavsson M, Jansen A-K, Martinelle M and Enfors
S-O (2003). Analysis and control of proteolysis of a fusion protein in Pichia
pastoris fed batch processes. J. Biotechnol. 102: 45-53. |
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Branneby C, Carlquist P, Magnusson A,
Hult K, Brinck T and Berglund P (2003). Carbon-carbon bonds by hydrolytic
enzymes. J. Am. Chem. Soc. 125: 874-875. |
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